Evaluating the immune responses of mice to subcutaneous immunization with Helicobacter pylori urease B subunit
نویسندگان
چکیده
منابع مشابه
Oral immunization of mice with vaccine of attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit.
OBJECTIVE To prepare the live recombinant vaccine of attenuated Salmonella typhimurium SL3261 expressing Helicobacter pylori (H. pylori) B subunit (UreB) and to determine whether it could be used as an oral vaccine against H. pylori infection. METHODS Using genomic DNA of H. pylori Sydney strain (SSI) as template, the H. pylori UreB gene fragment was amplified by PCR and subcloned into the ex...
متن کاملOral immunization of mice with lactic acid bacteria producing Helicobacter pylori urease B subunit partially protects against challenge with Helicobacter felis.
BACKGROUND The development of an efficacious vaccine against infection with Helicobacter pylori, the causative agent of chronic gastritis, peptic ulcer disease, and gastric adenocarcinoma, remains a challenge. Since the use of mucosal adjuvants is limited in human application, we have evaluated the potential of recombinant Lactobacillus strains producing H. pylori urease B (UreB) subunit to del...
متن کاملCloning and Expression of Recombinant Helicobacter pylori Urease A and B Subunits as a Putative Vaccine
Helicobacter pylori infection is among the most prevalent infections in the world involving more than half the adult population. H. pylori infection results in active chronic gastritis, peptic ulcers and enhances the risk of gastric malignancies. It is of utmost importance to prevent H. pylori infection particularly in highly prevalent countries including Iran. The urease holoenzyme produced by...
متن کاملImmunization with the immunodominant Helicobacter suis urease subunit B induces partial protection against H. suis infection in a mouse model
Helicobacter (H.) suis is a porcine and human gastric pathogen. Previous studies in mice showed that an H. suis infection does not result in protective immunity, whereas immunization with H. suis whole-cell lysate (lysate) protects against a subsequent experimental infection. Therefore, two-dimensional gel electrophoresis of H. suis proteins was performed followed by immunoblotting with pooled ...
متن کاملImmunization of Mice with Urease Vaccine Affords Protection against Helicobacter pylori Infection in the Absence of Antibodies and Is Mediated by MHC Class II–restricted Responses
We examined the roles of cell- and antibody-mediated immunity in urease vaccine-induced protection against Helicobacter pylori infection. Normal and knockout mice deficient in major histocompatibility complex (MHC) class I, MHC class II, or B cell responses were mucosally immunized with urease plus Escherichia coli heat-labile enterotoxin (LT), or parenterally immunized with urease plus aluminu...
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ژورنال
عنوان ژورنال: Journal of Animal Science and Biotechnology
سال: 2014
ISSN: 2049-1891
DOI: 10.1186/2049-1891-5-14